Keratin contaminations

Afin de maximiser les chances de succès des analyses de protéines  en spectrométrie de masse, il est vivement conseillé de préparer les échantillons selon les recommandations ci-dessous.

You: Keratins come from skin, hair and clothing.

• Wearing a clean coat and gloves is essential throughout the manipulation.
• Gloves must be renewed for each manipulation.
• Avoid wearing sweaters made of wool, mohair, etc.

The material: Keratins come from dust in suspension.

• Equipment and dishes that come into contact with the gel should be washed with detergent, rinsed with ultrapure water and dried with ethanol.
• All equipment should be stored away from dust.
• If possible, handle under a laminar flow hood.

The solutions: The solutions themselves can be a source of keratins.

• Preferably use 1.5 ml transparent polypropylene microtubes.
• Use new products that will be exclusively dedicated to gels.
• The cones will be reserved for proteomic use. Wearing gloves!
• Preferably use pre-filled tip boxes to avoid any manipulation of the pipette tips.

Preferably use pre-filled tip boxes to avoid any manipulation of the pipette tips.

• If possible, use thin gels (0.75mm).
• For 1D gels, load the empty wells with Laemmli buffer to limit the diffusion of proteins on the sides during migration.
• Conservation of the gel in 1% acetic acid at 4°C.
• Prefer Coomassie blue staining to silver nitrate staining.
• In the event of staining with silver nitrate, process the samples as soon as possible.