{"id":93,"date":"2022-10-19T16:27:30","date_gmt":"2022-10-19T14:27:30","guid":{"rendered":"http:\/\/proteome.u-bordeaux.fr\/?page_id=93"},"modified":"2022-10-28T15:00:54","modified_gmt":"2022-10-28T13:00:54","slug":"expertise","status":"publish","type":"page","link":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/","title":{"rendered":"Skills"},"content":{"rendered":"<p class=\"wp-block-paragraph\">Non-exhaustive list of services offered by the Bordeaux Proteome Platform.&nbsp;<br>For other types of applications, please contact the platform manager, <a href=\"mailto:caroline.tokarski@u-bordeaux.fr\">Caroline Tokarski<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Sample preparation for Mass Spectrometry analysis<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Electrophoretic or chromatographic separation of proteins\/peptides (SDS-PAGE, 2D-PAGE, OffGel, GelFree, C18, C4, SEC, IEX, monolithic\u2026).<\/li><li>Enrichment\/depletion of proteins\/peptides (sera analysis, plasmas, etc.).<\/li><li>Enrichment of peptide subspecies (phosphoproteomics, glycosylation\u2026).<\/li><li>Proteolysis adapted to the sample and the analysis (<em>in gel<\/em>, en solution, strat\u00e9gie multi-enzymatique, \u00e9chantillons \u00e0 l\u2019\u00e9tat de traces \u2026).<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Analysis of proteins\/peptides by High Resolution Mass Spectrometry<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Identification of proteins in complex mixtures.<\/li><li>Characterization of proteins: measurement of molecular mass, identification and localization of co\/post-translational modifications (phosphorylation, glycosylation, ubiquitination, etc.).<\/li><li>de novo sequencing (no database comparsion) <em>de novo<\/em> de prot\u00e9ines.<\/li><li>Protein de novo sequencing<\/li><li>Differential expression proteomics with labeling (SILAC, TMT) or without labeling (Label-free).<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Bio-informatics data processing<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Reprocessing of mass spectrometry data for the identification, characterization and quantification of proteins using dedicated software.<\/li><li>Partnership with the Bordeaux Bioinformatics Center for the analysis and integration of omics data: statistical validation, enrichment in GO terms, tools for visualization and interpretation of results, functional annotation of proteomes.<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">R&amp;D expertises<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Structural analysis of proteins by isotopic exchange or cross-linking: conformational study, protein-protein interactions, epitope mapping, etc.<\/li><li class=\"translation-block\">Imaging by MALDI-Orbitrap mass spectrometry: identification and localization of lipids, metabolites, peptides\/proteins (in situ digestion)\u2026<\/li><li>Structural analysis of proteins by isotopic exchange or cross-linking: conformational study, protein-protein interactions, epitope mapping, etc.<\/li><li>Top-down proteomic analysis: identification of intact proteins and their post-translational or chemical modifications, identification of protein variants, identification of cleaved protein forms <em>in situ<\/em>.<\/li><li>Analysis of glycoproteins\/glycopeptides: identification and localization of N- and O-glycosylations<\/li><li>Trace and ultra-trace analysis in the field of cultural heritage: identification of proteins, lipids and sugars, chemical\/post-translational modifications, original biological species from different types of ancient materials.<\/li><\/ul>","protected":false},"excerpt":{"rendered":"<p>Liste non exhaustive des prestations propos\u00e9es par la Plateforme Bordeaux Proteome.&nbsp;Pour d\u2019autres types d\u2019applications, contacter SVP la responsable de la plateforme, Caroline Tokarski. Pr\u00e9paration des \u00e9chantillons pour l&rsquo;analyse en Spectrom\u00e9trie de Masse S\u00e9paration \u00e9lectrophor\u00e9tique ou chromatographique des prot\u00e9ines\/peptides (SDS-PAGE, 2D-PAGE, OffGel, GelFree, C18, C4, SEC, IEX, monolithique \u2026). Enrichissement\/d\u00e9pl\u00e9tion des [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-93","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.9 - aioseo.com -->\n\t<meta name=\"description\" content=\"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/\" \/>\n\t<meta name=\"generator\" content=\"All in One SEO (AIOSEO) 4.9.9\" \/>\n\t\t<meta property=\"og:locale\" content=\"en_US\" \/>\n\t\t<meta property=\"og:site_name\" content=\"Bordeaux Proteome\" \/>\n\t\t<meta property=\"og:type\" content=\"article\" \/>\n\t\t<meta property=\"og:title\" content=\"Bordeaux Proteome - Expertise\" \/>\n\t\t<meta property=\"og:description\" content=\"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.\" \/>\n\t\t<meta property=\"og:url\" content=\"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/\" \/>\n\t\t<meta property=\"og:image\" content=\"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg\" \/>\n\t\t<meta property=\"og:image:secure_url\" content=\"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg\" \/>\n\t\t<meta property=\"article:published_time\" content=\"2022-10-19T14:27:30+00:00\" \/>\n\t\t<meta property=\"article:modified_time\" content=\"2022-10-28T13:00:54+00:00\" \/>\n\t\t<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n\t\t<meta name=\"twitter:title\" content=\"Bordeaux Proteome - Expertise\" \/>\n\t\t<meta name=\"twitter:description\" content=\"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.\" \/>\n\t\t<meta name=\"twitter:image\" content=\"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg\" \/>\n\t\t<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t\t<meta name=\"twitter:data1\" content=\"proteome\" \/>\n\t\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n\t\t<script type=\"application\/ld+json\" class=\"aioseo-schema\">\n\t\t\t{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#breadcrumblist\",\"itemListElement\":[{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en#listItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\",\"nextItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#listItem\",\"name\":\"Expertise\"}},{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#listItem\",\"position\":2,\"name\":\"Expertise\",\"previousItem\":{\"@type\":\"ListItem\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en#listItem\",\"name\":\"Accueil\"}}]},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/#organization\",\"name\":\"Bordeaux Proteome\",\"description\":\"La plateforme prot\\u00e9omique au service de toutes les communaut\\u00e9s\",\"url\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"url\":\"https:\\\/\\\/i0.wp.com\\\/proteome.u-bordeaux.fr\\\/wp-content\\\/uploads\\\/2024\\\/01\\\/cropped-LOGO-Bordeaux-Proteome-Bordeaux_Orange-long_small.png?fit=200%2C64&ssl=1\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#organizationLogo\",\"width\":200,\"height\":64,\"caption\":\"Logo Bordeaux Proteome\"},\"image\":{\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#organizationLogo\"},\"sameAs\":[\"https:\\\/\\\/www.linkedin.com\\\/company\\\/proteome-plateform-bordeaux\\\/\"]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#webpage\",\"url\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/\",\"name\":\"Bordeaux Proteome - Expertise\",\"description\":\"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.\",\"inLanguage\":\"en-US\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/#website\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/expertise\\\/#breadcrumblist\"},\"datePublished\":\"2022-10-19T16:27:30+02:00\",\"dateModified\":\"2022-10-28T15:00:54+02:00\"},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/\",\"name\":\"Bordeaux Proteome\",\"description\":\"La plateforme prot\\u00e9omique au service de toutes les communaut\\u00e9s\",\"inLanguage\":\"en-US\",\"publisher\":{\"@id\":\"https:\\\/\\\/proteome.u-bordeaux.fr\\\/en\\\/#organization\"}}]}\n\t\t<\/script>\n\t\t<!-- All in One SEO -->\n\n","aioseo_head_json":{"title":"Bordeaux Proteome - Expertise","description":"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.","canonical_url":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/","robots":"max-image-preview:large","keywords":"","webmasterTools":{"miscellaneous":""},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"BreadcrumbList","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#breadcrumblist","itemListElement":[{"@type":"ListItem","@id":"https:\/\/proteome.u-bordeaux.fr\/en#listItem","position":1,"name":"Accueil","item":"https:\/\/proteome.u-bordeaux.fr\/en","nextItem":{"@type":"ListItem","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#listItem","name":"Expertise"}},{"@type":"ListItem","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#listItem","position":2,"name":"Expertise","previousItem":{"@type":"ListItem","@id":"https:\/\/proteome.u-bordeaux.fr\/en#listItem","name":"Accueil"}}]},{"@type":"Organization","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/#organization","name":"Bordeaux Proteome","description":"La plateforme prot\u00e9omique au service de toutes les communaut\u00e9s","url":"https:\/\/proteome.u-bordeaux.fr\/en\/","logo":{"@type":"ImageObject","url":"https:\/\/i0.wp.com\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2024\/01\/cropped-LOGO-Bordeaux-Proteome-Bordeaux_Orange-long_small.png?fit=200%2C64&ssl=1","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#organizationLogo","width":200,"height":64,"caption":"Logo Bordeaux Proteome"},"image":{"@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#organizationLogo"},"sameAs":["https:\/\/www.linkedin.com\/company\/proteome-plateform-bordeaux\/"]},{"@type":"WebPage","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#webpage","url":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/","name":"Bordeaux Proteome - Expertise","description":"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.","inLanguage":"en-US","isPartOf":{"@id":"https:\/\/proteome.u-bordeaux.fr\/en\/#website"},"breadcrumb":{"@id":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/#breadcrumblist"},"datePublished":"2022-10-19T16:27:30+02:00","dateModified":"2022-10-28T15:00:54+02:00"},{"@type":"WebSite","@id":"https:\/\/proteome.u-bordeaux.fr\/en\/#website","url":"https:\/\/proteome.u-bordeaux.fr\/en\/","name":"Bordeaux Proteome","description":"La plateforme prot\u00e9omique au service de toutes les communaut\u00e9s","inLanguage":"en-US","publisher":{"@id":"https:\/\/proteome.u-bordeaux.fr\/en\/#organization"}}]},"og:locale":"en_US","og:site_name":"Bordeaux Proteome","og:type":"article","og:title":"Bordeaux Proteome - Expertise","og:description":"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.","og:url":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/","og:image":"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg","og:image:secure_url":"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg","article:published_time":"2022-10-19T14:27:30+00:00","article:modified_time":"2022-10-28T13:00:54+00:00","twitter:card":"summary_large_image","twitter:title":"Bordeaux Proteome - Expertise","twitter:description":"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.","twitter:image":"https:\/\/proteome.u-bordeaux.fr\/wp-content\/uploads\/2023\/02\/LOGO-Bordeaux-Proteome-Bordeaux_Orange-long-scaled.jpg","twitter:label1":"Written by","twitter:data1":"proteome","twitter:label2":"Est. reading time","twitter:data2":"2 minutes"},"aioseo_meta_data":{"post_id":"93","title":null,"description":"A scientific and technological platform open to all academic and private scientific communities through the provision of services, materials and expertise in the field of proteomic analysis.","keywords":null,"keyphrases":null,"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"default","og_image_type":"content","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":null,"og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":true,"twitter_card":"default","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":[],"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"","isEnabled":true},"graphs":[]},"schema_type":"default","schema_type_options":null,"pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":null,"robots_max_videopreview":null,"robots_max_imagepreview":"large","priority":null,"frequency":null,"local_seo":null,"breadcrumb_settings":null,"limit_modified_date":false,"ai":null,"created":"2023-04-26 10:49:20","updated":"2025-06-06 15:31:53","seo_analyzer_scan_date":null},"aioseo_breadcrumb":"<div class=\"aioseo-breadcrumbs\"><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/proteome.u-bordeaux.fr\/en\" title=\"Accueil\">Accueil<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">&raquo;<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\tExpertise\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Accueil","link":"https:\/\/proteome.u-bordeaux.fr\/en"},{"label":"Expertise","link":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/"}],"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/comments?post=93"}],"version-history":[{"count":13,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93\/revisions"}],"predecessor-version":[{"id":562,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93\/revisions\/562"}],"wp:attachment":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/media?parent=93"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}