{"id":93,"date":"2022-10-19T16:27:30","date_gmt":"2022-10-19T14:27:30","guid":{"rendered":"http:\/\/proteome.u-bordeaux.fr\/?page_id=93"},"modified":"2022-10-28T15:00:54","modified_gmt":"2022-10-28T13:00:54","slug":"expertise","status":"publish","type":"page","link":"https:\/\/proteome.u-bordeaux.fr\/en\/expertise\/","title":{"rendered":"Skills"},"content":{"rendered":"<p>Non-exhaustive list of services offered by the Bordeaux Proteome Platform.&nbsp;<br>For other types of applications, please contact the platform manager, <a href=\"mailto:caroline.tokarski@u-bordeaux.fr\">Caroline Tokarski<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Sample preparation for Mass Spectrometry analysis<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Electrophoretic or chromatographic separation of proteins\/peptides (SDS-PAGE, 2D-PAGE, OffGel, GelFree, C18, C4, SEC, IEX, monolithic\u2026).<\/li><li>Enrichment\/depletion of proteins\/peptides (sera analysis, plasmas, etc.).<\/li><li>Enrichment of peptide subspecies (phosphoproteomics, glycosylation\u2026).<\/li><li>Proteolysis adapted to the sample and the analysis (<em>in gel<\/em>, en solution, strat\u00e9gie multi-enzymatique, \u00e9chantillons \u00e0 l\u2019\u00e9tat de traces \u2026).<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Analysis of proteins\/peptides by High Resolution Mass Spectrometry<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Identification of proteins in complex mixtures.<\/li><li>Characterization of proteins: measurement of molecular mass, identification and localization of co\/post-translational modifications (phosphorylation, glycosylation, ubiquitination, etc.).<\/li><li>de novo sequencing (no database comparsion) <em>de novo<\/em> de prot\u00e9ines.<\/li><li>Protein de novo sequencing<\/li><li>Differential expression proteomics with labeling (SILAC, TMT) or without labeling (Label-free).<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Bio-informatics data processing<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Reprocessing of mass spectrometry data for the identification, characterization and quantification of proteins using dedicated software.<\/li><li>Partnership with the Bordeaux Bioinformatics Center for the analysis and integration of omics data: statistical validation, enrichment in GO terms, tools for visualization and interpretation of results, functional annotation of proteomes.<\/li><\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">R&amp;D expertises<\/h4>\n\n\n\n<ul class=\"wp-block-list\" style=\"font-size:16px\"><li>Structural analysis of proteins by isotopic exchange or cross-linking: conformational study, protein-protein interactions, epitope mapping, etc.<\/li><li class=\"translation-block\">Imaging by MALDI-Orbitrap mass spectrometry: identification and localization of lipids, metabolites, peptides\/proteins (in situ digestion)\u2026<\/li><li>Structural analysis of proteins by isotopic exchange or cross-linking: conformational study, protein-protein interactions, epitope mapping, etc.<\/li><li>Top-down proteomic analysis: identification of intact proteins and their post-translational or chemical modifications, identification of protein variants, identification of cleaved protein forms <em>in situ<\/em>.<\/li><li>Analysis of glycoproteins\/glycopeptides: identification and localization of N- and O-glycosylations<\/li><li>Trace and ultra-trace analysis in the field of cultural heritage: identification of proteins, lipids and sugars, chemical\/post-translational modifications, original biological species from different types of ancient materials.<\/li><\/ul>","protected":false},"excerpt":{"rendered":"<p>Liste non exhaustive des prestations propos\u00e9es par la Plateforme Bordeaux Proteome.&nbsp;Pour d\u2019autres types d\u2019applications, contacter SVP la responsable de la plateforme, Caroline Tokarski. Pr\u00e9paration des \u00e9chantillons pour l&rsquo;analyse en Spectrom\u00e9trie de Masse S\u00e9paration \u00e9lectrophor\u00e9tique ou chromatographique des prot\u00e9ines\/peptides (SDS-PAGE, 2D-PAGE, OffGel, GelFree, C18, C4, SEC, IEX, monolithique \u2026). Enrichissement\/d\u00e9pl\u00e9tion des [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-93","page","type-page","status-publish","hentry"],"aioseo_notices":[],"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/comments?post=93"}],"version-history":[{"count":13,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93\/revisions"}],"predecessor-version":[{"id":562,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/93\/revisions\/562"}],"wp:attachment":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/media?parent=93"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}