{"id":419,"date":"2022-10-24T14:06:38","date_gmt":"2022-10-24T12:06:38","guid":{"rendered":"http:\/\/proteome.u-bordeaux.fr\/?page_id=419"},"modified":"2026-01-02T14:26:11","modified_gmt":"2026-01-02T13:26:11","slug":"conseils","status":"publish","type":"page","link":"https:\/\/proteome.u-bordeaux.fr\/en\/conseils\/","title":{"rendered":"Conseils pratiques pour une pr\u00e9paration d&rsquo;\u00e9chantillon optimale"},"content":{"rendered":"<h1 class=\"wp-block-heading\">Keratin contaminations<\/h1>\n\n\n\n<p class=\"justification_texte\">Afin de maximiser les chances de succ\u00e8s des analyses de prot\u00e9ines \u00a0en spectrom\u00e9trie de masse, il est vivement conseill\u00e9 de pr\u00e9parer les \u00e9chantillons selon les recommandations ci-dessous.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">You: Keratins come from skin, hair and clothing.<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Wearing a clean coat and gloves is essential throughout the manipulation.<\/li>\n\n\n\n<li>Gloves must be renewed for each manipulation.<\/li>\n\n\n\n<li>Avoid wearing sweaters made of wool, mohair, etc.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">The material: Keratins come from dust in suspension.<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Equipment and dishes that come into contact with the gel should be washed with detergent, rinsed with ultrapure water and dried with ethanol.<\/li>\n\n\n\n<li>All equipment should be stored away from dust.<\/li>\n\n\n\n<li>If possible, handle under a laminar flow hood.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">The solutions: The solutions themselves can be a source of keratins.<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Preferably use 1.5 ml transparent polypropylene microtubes.<\/li>\n\n\n\n<li>Use new products that will be exclusively dedicated to gels.<\/li>\n\n\n\n<li>The cones will be reserved for proteomic use. Wearing gloves!<\/li>\n\n\n\n<li>Preferably use pre-filled tip boxes to avoid any manipulation of the pipette tips.<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">Preferably use pre-filled tip boxes to avoid any manipulation of the pipette tips.<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>If possible, use thin gels (0.75mm).<\/li>\n\n\n\n<li>For 1D gels, load the empty wells with Laemmli buffer to limit the diffusion of proteins on the sides during migration.<\/li>\n\n\n\n<li>Conservation of the gel in 1% acetic acid at 4\u00b0C.<\/li>\n\n\n\n<li>Prefer Coomassie blue staining to silver nitrate staining.<\/li>\n\n\n\n<li>In the event of staining with silver nitrate, process the samples as soon as possible.<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"<p>Les contaminations keratine Afin de maximiser les chances de succ\u00e8s des analyses de prot\u00e9ines \u00a0en spectrom\u00e9trie de masse, il est vivement conseill\u00e9 de pr\u00e9parer les \u00e9chantillons selon les recommandations ci-dessous. Vous : Les k\u00e9ratines viennent de la peau, des cheveux et des v\u00eatements. Le mat\u00e9riel : Les k\u00e9ratines proviennent des [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-419","page","type-page","status-publish","hentry"],"aioseo_notices":[],"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/419","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/comments?post=419"}],"version-history":[{"count":6,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/419\/revisions"}],"predecessor-version":[{"id":2181,"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/pages\/419\/revisions\/2181"}],"wp:attachment":[{"href":"https:\/\/proteome.u-bordeaux.fr\/en\/wp-json\/wp\/v2\/media?parent=419"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}